mca1957 f4 80 cl  (Bio-Rad)

Journal: Scientific Reports

Article Title: The pharmaceutical solvent N-methyl-2-pyrollidone (NMP) attenuates inflammation through Krüppel-like factor 2 activation to reduce atherogenesis

doi: 10.1038/s41598-020-68350-2

Figure Lengend Snippet: NMP treatment reduces macrophages infiltration and inflammation. ( a ) Representative immunostaining of macrophages, smooth muscle cells (SMC) and activated endothelium in aortic sinus lesions of NMP treated and untreated C57BL/6 J mice fed with HFD for 12 weeks. Lesions were stained for biomarkers of macrophages (CD68; yellow), SMCs (α-actinin; green) and stressed endothelium (Vcam-1; red); nuclei were stained with DAPI (blue). Merged images are also shown. Lm, aortic lumen, M, tunica media, and I, tunica intima. White dashed lines demarcate the elastic lamina. Bars, 200 μm. ( b ) Vcam-1 and merge staining as in ( a ) from different animals. ( c ) Quantitative analysis of relative Vcam-1 immunostaining relative to the aortic sections size from ( b ).

Article Snippet: Immunofluorescence samples were stained with rat anti-mouse CD68 F4/80 (AbD Serotec, MCA497R) and Cy3-conjugated mouse anti-smooth muscle actin (SMA) (Sigma-Aldrich, C6198).

Techniques: Immunostaining, Staining

Journal: Oncogene

Article Title: Cellular features of senescence during the evolution of human and murine ductal pancreatic cancer

doi: 10.1038/onc.2011.350

Figure Lengend Snippet: Table of primary antibodies used

Article Snippet: Error bars indicate s.e.m. table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Species Supplier Dilution Tissue origin p16 Rabbit Santa Cruz M156, sc-1207 (Santa Cruz, CA, USA) 1:500 M p19 Rabbit Abcam ab80 (Cambridge, UK) 1:2000 M DcR2 Rabbit Stressgen AAP-371 # 03160608 (Exeter, UK) 1:500 M, H Dec1 Rabbit Adrian Harris (Oxford, UK) 1:100 M, H p15 Rabbit Marcos Malumbres (Madrid, Spain) 1:1000 M, H γH2AX (Ser139) Mouse Upstate Cell Signalling Solutions 1:500 M, H 05-636 Lot# 32526 Clone JBW301 (Billerica, MA, USA) p53 Mouse BioVision 3036-100 (Mountainview, CA, USA) 1:100 M p21 Mouse Santa Cruz Biotechnology (F-5) sc-6246 1:500 M GFP Chicken Abcam ab 13970 1:2000 M Ki67 Rat DakoCytomation Clone TEC-3 M7249 (Ely, UK) 1:25 M Cleaved Caspase 3 (Asp175) Rabbit Cell Signalling 9664 (Danvers, MA, USA) 1:100 M Smooth muscle actin Mouse DakoCytomation Clone 1A4 M0851 1:200 M, H F4/80 Rat Serotec MCA1957 (Kidlington, UK) 1:100 M MCM2 Goat Santa Cruz Biotechnology (N-19) sc-9839 1:100 H p16 Mouse Santa Cruz Biotechnology (F-12) sc-1661 1:200 H p15 Rabbit Cell Signalling 4822 1:100 H p14 Rabbit Abcam ab3642 1:100 H CD68 Mouse DakoCytomation Clone PG-M1 M0876 1:100 H Open in a separate window Abbreviations: H, human; M, mouse.

Techniques: